Journal: Cell & Bioscience

Article Title: Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma

doi: 10.1186/s13578-022-00785-8

Figure Lengend Snippet: Expression pattern of KMT2D in patients with OSCC. A Representative immunohistochemical staining of KMT2D in normal mucosa and primary OSCC tissue of KMT2D(+) and KMT2D(−). (scale bars = 100 μm). B Quantification analysis of immunohistochemical histoscore of KMT2D among normal mucosa and OSCC. C KMT2D expression levels in OSCC tissues from different pathological grades. D KMT2D expression levels in OSCC tissues from different tumor sizes. E KMT2D mRNA expression levels of paired normal mucosa and OSCC from TCGA datasets. F KMT2D protein expression levels in different cell lines. G KMT2D protein expression in human OSCC tissues and adjacent normal mucosa from the same patient. Results are representative of at least three independent experiments (*p < 0.05, **p < 0.01). Data are presented as means ± SD. OSCC, oral squamous cell carcinoma; SD standard deviation, ns no significant, KMT2D(+) KMT2D positive, KMT2D(−) KMT2D negative

Article Snippet: The OSCC cell lines CAL27, SCC25 and SCC4 were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: Expressing, Immunohistochemical staining, Staining, Standard Deviation

Journal: Cell & Bioscience

Article Title: Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma

doi: 10.1186/s13578-022-00785-8

Figure Lengend Snippet: KMT2D contributes to the self-renewal ability of primary OSCC cells. A Representative images showed the immunofluorescence staining of KMT2D in patient-derived organoids generated from OSCC and paracancerous specimens, as well as their parental tissues. (scale bars = 50 μm). B Representative images indicated the organoid reconstitution assay (i.e. organoid morphology and clone size) conducted for OSCC cells transfected with Sh-CON and Sh-KMT2D. C Quantificational analysis showed the organoid reconstitution assay (i.e. organoid forming efficiency) conducted for OSCC cells transfected with Sh-KMT2D and Sh-CON. Student's t-test. D Representative images showed the immunofluorescence staining of β-catenin and CD133 in patient-derived OSCC organoids transfected with Sh-CON and Sh-KMT2D. (scale bars = 50 μm) Results are representative of at least three independent experiments(*p < 0.05,**p < 0.01, and ***p < 0.001). Data are presented as means ± SD. OSCC oral squamous cell carcinoma, SD standard deviation

Article Snippet: The OSCC cell lines CAL27, SCC25 and SCC4 were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: Immunofluorescence, Staining, Derivative Assay, Generated, Reconstitution Assay, Transfection, Standard Deviation

Journal: Cell & Bioscience

Article Title: Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma

doi: 10.1186/s13578-022-00785-8

Figure Lengend Snippet: Expression of KMT2D aggravates malignant behaviors of OSCC cells. A Immunoblotting of KMT2D in SCC4, SCC25, and CAL27 cells respectively transfected with Sh-CON and Sh-KMT2D. B Colony formation assays and quantification analysis generated from SCC4 and SCC25 cells transfected with Sh-CON and Sh-KMT2D. C Sphere formation assays and quantification analysis carried out from SCC4, SCC25, and CAL27 cells transfected with Sh-CON and Sh-KMT2D. (scale bars = 50 μm). D Wound healing assays and quantification analysis generated from SCC4, SCC25, and CAL27 cells transfected with Sh-CON and Sh-KMT2D. (scale bars = 50 μm). E Invasion assays and quantification analysis generated from SCC4, SCC25 cells transfected with Sh-CON and Sh-KMT2D. (scale bars = 50 μm). Results are representative of at least three independent experiments (*p < 0.05, **p < 0.01 and ***p < 0.001). Data are presented as means ± SD; SD standard deviation

Article Snippet: The OSCC cell lines CAL27, SCC25 and SCC4 were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: Expressing, Western Blot, Transfection, Generated, Standard Deviation

Journal: Cell & Bioscience

Article Title: Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma

doi: 10.1186/s13578-022-00785-8

Figure Lengend Snippet: Knockdown of KMT2D impairs OSCC tumor growth in vivo . A Images of BALB/c nude mice from the two groups. B Images of xenograft tumors excised from BALB/c nude mice. C Quantification analysis of volumes of the xenograft tumors measured every 7 days on BALB/c nude mice. n = 8. D Weights of xenograft tumors excised from the BALB/c nude mice were evaluated. n = 8. E Representative hematoxylin and eosin (HE) and immunohistochemical staining of KMT2D and CSC-related markers in xenograft tumors. (scale bars = 50 μm). Results are representative of at least three independent experiments (*p < 0.05). CS cancer stem cell

Article Snippet: The OSCC cell lines CAL27, SCC25 and SCC4 were obtained from the China Center for Type Culture Collection (Shanghai, China).

Techniques: Knockdown, In Vivo, Immunohistochemical staining, Staining